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antibax  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc antibax
    Antibax, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 98/100, based on 9382 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/antibax/Bax+Antibody/pm41450030-113-21-23
    Average 98 stars, based on 9382 article reviews
    antibax - by Bioz Stars, 2026-09
    98/100 stars

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    Related Articles

    Incubation:

    Article Title: Melatonin Ameliorates Heat Stress-Induced Oxidative Apoptosis in Mouse Spermatocytes via Autophagy and Ferroptosis Pathways.
    Article Snippet: .. The sections were incubated overnight at 4°C with the following primary antibodies: anti-Sycp3 (1:200, Proteintech), antiBax (1:200, Cell Signaling Technology), and anti-Gpx4 (1:200, Affinity) 4°C, and then biotinylated rabbit anti-mouse antibody was added. .. Immunoreactivity was detected by indirect immunoperoxidase staining (Vector Labs, Newark, CA, USA) with DAB.

    Article Title: Potential of CLSPN as a therapeutic target in melanoma: a key player in melanoma progression and tumor microenvironment.
    Article Snippet: Afterwards, 10% SDS-PAGE was applied to separate proteins, and the proteins were transferred to polyvinylidene difluoride membranes (Millipore, USA). .. The membranes were blocked using 5% non-fat milk for 1 h, incubated overnight with primary antibodies against CLSPN (Affinity, DF7525), IFI44L (SAB, 27948), p-STAT1 (Proteintech, 28977-1-AP), STAT1 (Proteintech, 10144-2-AP), XAF1 (BOSTER, BA4986), GAPDH (Affinity, AF7021), antiBax (Cell Signaling Technology, #5023), anti-Bcl-2 (Cell Signaling Technology, #3498), anti-cleaved-PARP (Cell Signaling Technology, #5625T), anti-cleaved-caspase 9 (Cell Signaling Technology, #20750), anti-β-tubulin (Affinity, AF7011), anti-β-actin (Affinity, #AF7018) at 4 °C, and then incubated for 1 h with secondary antibody goat Anti-Rabbit IgG (Affinity, #S0001). .. Membranes were treated with Pierce ECL Plus Western Blotting Substrate (Thermo Fisher Scientific, USA).

    Article Title: The cell cycle regulator p16 promotes tumor infiltrated CD8 + T cell exhaustion and apoptosis.
    Article Snippet: The protein samples were then separated by SDS–PAGE gels and transferred onto Nitrocellulose membranes (Bio-Rad). .. After blocking with 5% nonfat milk in PBST, the membranes were incubated with primary antibodies against anti-BCL-XL (CST, 2764) or antiBAX (CST, 14796) overnight at 4 °C, followed by goat anti-rabbit HRPcoupled secondary antibody (Absin, abs20040ss) for 2 h at room temperature. ..

    Article Title: Ubiquitin-specific protease 14 inhibition promotes mitophagy and attenuates neural apoptosis after spinal cord injury.
    Article Snippet: .. Membranes were blocked with 5% non-fat dry milk (BioFroxx, Germany) in Tris-buffered saline with 0.1% Tween-20 (TBST) for 3 h at room temperature and then incubated overnight at 4 °C with primary antibodies against the following proteins: anti-USP14 (1:2000, Proteintech, 14517-1-AP), antiBax (1:1000, CST, #5023), anti-Bcl2 (1:1000, Abcam, ab692), anti-Caspase-3 (1:1000, CST, #9662), anti-Cleaved Caspase-3 (1:1000, CST, #9664), anti-Caspase-9 (1:1000, CST, #9508), anti-Cleaved Caspase-9 (1:1000, CST, #20750), anti-LC3B (1:1000, Abcam, ab192890), anti-p62 (1:1000, CST, #8025), anti-TOM20 (1:1000, Abcam, ab56783), anti-ATPB (1:2000, Abcam, ab14730), anti-β-actin(1:20000, Proteintech, 66009-1-Ig). .. After washing with TBST, membranes were incubated with horseradish peroxidase (HRP)-conjugated secondary antibodies (1:5000, Dingguo Biotechnology, China) for 1 h at room temperature.

    Article Title: Structural characteristics and anti-tumor activities of a novel polysaccharide from Klebsiella sp. SXW12.
    Article Snippet: The purpose of this study is to elucidate the structure and biological properties of an extracellular polysaccharide (EPS), named EPS12, produced by Klebsiella sp. SXW12, a strain isolated from pond sludge.. The maximum EPS12 yield of 15 g/L was obtained after optimizing the fermentation conditions by single-factor effects.. EPS12 was an acidic homogeneous polysaccharide, and the molecular weight was measured to be 9.39 × 104 Da.

    Western Blot:

    Article Title: Structure-activity relationships reveal a 4-(2-pyridyl)chalcone as a potent cell death inducer.
    Article Snippet: .. The primary antibodies used for immunoblots were obtained from the following companies (all at 1:1000 dilution): anticaspase-3 (#ADI-AAP-113) was from Enzo (Ann Arbor, MI, USA); anticaspase-9 (#9502), anti-caspase-8 (#9746), anti-Bcl-2 (#4223), antiBax (#2772), anti-phospho-JNK/SAPK (phosphor T183 + Y185) (#9251), anti-JNK/SAPK (#9252), anti-phospho-p44/42 MAPK (Erk1/ 2) (Thr202/Tyr204) (#9101), anti-p44/42 MAP Kinase (ERK1/2) (#9102), anti-phospho-p38MAPK (T180/Y182) (#9211), anti-p38MAPK (#9212), and anti-α-tubulin (#2125) antibodies from Cell Signaling Technology (Beverly, MA, USA). .. Anti-PARP [poly(ADP-ribose) polymerase] (#551024) and anti-cytochrome c (#556433) were from BD Pharmingen (San Diego, CA, USA); anti-DR4 (ab8414) was from Abcam (Cambridge, UK).

    Blocking Assay:

    Article Title: The cell cycle regulator p16 promotes tumor infiltrated CD8 + T cell exhaustion and apoptosis.
    Article Snippet: The protein samples were then separated by SDS–PAGE gels and transferred onto Nitrocellulose membranes (Bio-Rad). .. After blocking with 5% nonfat milk in PBST, the membranes were incubated with primary antibodies against anti-BCL-XL (CST, 2764) or antiBAX (CST, 14796) overnight at 4 °C, followed by goat anti-rabbit HRPcoupled secondary antibody (Absin, abs20040ss) for 2 h at room temperature. ..

    Article Title: Structural characteristics and anti-tumor activities of a novel polysaccharide from Klebsiella sp. SXW12.
    Article Snippet: The purpose of this study is to elucidate the structure and biological properties of an extracellular polysaccharide (EPS), named EPS12, produced by Klebsiella sp. SXW12, a strain isolated from pond sludge.. The maximum EPS12 yield of 15 g/L was obtained after optimizing the fermentation conditions by single-factor effects.. EPS12 was an acidic homogeneous polysaccharide, and the molecular weight was measured to be 9.39 × 104 Da.

    Saline:

    Article Title: Ubiquitin-specific protease 14 inhibition promotes mitophagy and attenuates neural apoptosis after spinal cord injury.
    Article Snippet: .. Membranes were blocked with 5% non-fat dry milk (BioFroxx, Germany) in Tris-buffered saline with 0.1% Tween-20 (TBST) for 3 h at room temperature and then incubated overnight at 4 °C with primary antibodies against the following proteins: anti-USP14 (1:2000, Proteintech, 14517-1-AP), antiBax (1:1000, CST, #5023), anti-Bcl2 (1:1000, Abcam, ab692), anti-Caspase-3 (1:1000, CST, #9662), anti-Cleaved Caspase-3 (1:1000, CST, #9664), anti-Caspase-9 (1:1000, CST, #9508), anti-Cleaved Caspase-9 (1:1000, CST, #20750), anti-LC3B (1:1000, Abcam, ab192890), anti-p62 (1:1000, CST, #8025), anti-TOM20 (1:1000, Abcam, ab56783), anti-ATPB (1:2000, Abcam, ab14730), anti-β-actin(1:20000, Proteintech, 66009-1-Ig). .. After washing with TBST, membranes were incubated with horseradish peroxidase (HRP)-conjugated secondary antibodies (1:5000, Dingguo Biotechnology, China) for 1 h at room temperature.



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